Effect of prolonged venostasis on lipid profile parameters among apparently healthy University students: A case-control study in Ghana

  • Richard K.D. Ephraim Department of Medical Laboratory Science, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast , Cape Coast, Ghana
  • Andrews A. Appiah Department of Medical Laboratory Science, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast , Cape Coast, Ghana
  • Samuel A. Sakyi Department of Molecular Medicine, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
  • Enoch O. Anto Department of Molecular Medicine, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Keywords: Venostasis, lipid profile, tourniquet, prolonged, application

Abstract

Background: The effect of prolonged tourniquet application on lipid profile parameters (total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-c) and low density lipoprotein cholesterol (LDL-c)) among healthy students in the University of Cape Coast was assessed in this study.
Methods: A total of 30 participants were sampled and venous blood was taken from both arms at different duration of tourniquet application. Blood was allowed to clot, centrifuged and serum separated and stored until assay. Lipid profile parameters were measured using the enzymatic techniques with the aid of a spectrophotometer. Lipid parameters were expressed in mmol/L. The relationship between the concentrations was analyzed using GraphPad Prism version 6.0.
Results: The mean serum levels of total cholesterol and HDL-c were significantly (p<0.05) elevated after long duration (13.44±2.40 s) of tourniquet application compared to short duration (11.20±0.38 s) of tourniquet application. Despite the fact that the serum levels of triglyceride and LDL-c increased after long duration of tourniquet application compared to short duration, the difference was not significant (p>0.05). There was significant % mean differences of 7.0% (p<0.0001) and 11.2% (p=0.0082) in the concentrations of total cholesterol and HDL-c respectively following prolonged tourniquet application of 120-180 s. A significant increase of up to 7% (5.01±0.04, 5.352±0.05, p<0.0001) and 11% (2.41±0.07, 2.674±0.07, p=0.0082) in the concentrations of total cholesterol and HDL-c respectively was also recorded.
Conclusion: Prolonged venostasis has a direct effect on the concentration of serum total cholesterol and HDL-c but not triglyceride and LDL-c. Nonapplication of tourniquet in patients with prominent veins, the application of standardized external pressure as well the early release of tourniquet after needle insertion in the vein should be followed during sample taking for lipid profile.
Annals of Medical Laboratory Science (2021) 1(1), 1 - 7

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Published
2021-03-20
How to Cite
Ephraim, R. K., Appiah, A. A., Sakyi, S. A., & Anto, E. O. (2021). Effect of prolonged venostasis on lipid profile parameters among apparently healthy University students: A case-control study in Ghana. Annals of Medical Laboratory Science, 1(1), 1-7. https://doi.org/10.51374/annalsmls.2021.1.1.0016
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Articles